Details

Eradikit™ EIAV is licensed by:

• Centro di Referenza Nazionale per l’Anemia Infettiva Equina (Roma)
• Friedrich-Loeffler-Institut (Zul. Nr. FLI-B 676)

Eradikit™ EIAV includes:

• plates are coated with a mix of gag and env peptides (for a total of 460 samples)

Short protocol

Protocol 1 (OIE validated)

• Sample incubation at 37°C
• Sample dilution: 1:20 (5µl of serum in 95µl of Serum Dilution Buffer)

Protocol 2

• Sample incubation at Room Temperature
• Sample dilution: 1:4 (25µl of serum in 75µl of Serum Dilution Buffer)

1. Samples are directly diluted into the wells and incubated 60’
2. After washing, the enzyme-labeled, anti-species globulin conjugate is added and incubated for 60’
3. After washing, the substrate is added and the colorimetric reaction is read at 405nm

Documents and Support

• MSDS

• Technical bulletin 10.2014 – Different incubation temperatures
• Technical bulletin 11.2014 – Test on lipemic and hemolytic sera